ps6-af488 antibody Search Results


ps6 ps235/ps236  (Becton Dickinson)
90
Becton Dickinson ps6 ps235/ps236
Ps6 Ps235/Ps236, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ps6-af488+antibody/s6+mouse+anti++ps235+ps236/pm36302964-316-13-18
Average 90 stars, based on 1 article reviews
ps6 ps235/ps236 - by Bioz Stars, 2026-10
90/100 stars
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phosflow antibodies  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc phosflow antibodies
The Na + /K + ATPase restrains tonic signaling and CD8 + T-cell dysfunction. A, Summary bar graphs quantifying fluorescence of the voltage-sensitive indicator DiSBAC 2 3, reflecting the transmembrane electrochemical gradient ( V m ) of CD8 + T cells in standard RPMI-1640 (Vehicle) or isotonic hyperkalemic conditions (K + 20 mmol/L) captured by flow cytometry following CRISPR-Cas9–mediated disruption (Scramble, sgRNA Atp1a1 ) or transduction with retroviral particles. OE, overexpression; DN, Dominant negative. B and C, Summary quantification of cytoplasmic Ca 2+ as interval Fluo-3/FuraRed fluorescence following TCR cross-linking ( B ) or ionomycin induced store-operated calcium flux ( C ) as indicated, captured by flow cytometry. Iono, Ionomycin. n = 3 technical replicates depicted, representative of two independent experiments. D, Representative <t>Phosflow</t> cytometry plots and summary quantification of live, singlet, CD8 + T-cell populations in the presence or absence of soluble TCR cross-linking stimulation for five minutes. E, Concatenated single-cell overlay and colorimetric density tSNE-based depiction of the indicated proteins captured by flow cytometry. F, Representative flow cytometry plots and summary quantification for the indicated markers in ex vivo expanded CD8 + T cells. G, Representative and summary quantification of cytokines following acute TCR restimulation of ex vivo expanded CD8 + T cells. Error bars represent standard deviation. *, P < 0.05; **, P < 0.005; ****, P < 0.001, two-tailed Student t tests ( A, D, F, and G ). ****, P < 0.001 for two-way ANOVA ( B and C ).
Phosflow Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ps6-af488+antibody/Phospho-S6+Ribosomal+Protein+(Ser235%2F236)+Antibody/pmc10765769-91-10-22
Average 96 stars, based on 1 article reviews
phosflow antibodies - by Bioz Stars, 2026-10
96/100 stars
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anti-ps6-af488 p235/p236 antibody  (Becton Dickinson)
90
Becton Dickinson anti-ps6-af488 p235/p236 antibody
The Na + /K + ATPase restrains tonic signaling and CD8 + T-cell dysfunction. A, Summary bar graphs quantifying fluorescence of the voltage-sensitive indicator DiSBAC 2 3, reflecting the transmembrane electrochemical gradient ( V m ) of CD8 + T cells in standard RPMI-1640 (Vehicle) or isotonic hyperkalemic conditions (K + 20 mmol/L) captured by flow cytometry following CRISPR-Cas9–mediated disruption (Scramble, sgRNA Atp1a1 ) or transduction with retroviral particles. OE, overexpression; DN, Dominant negative. B and C, Summary quantification of cytoplasmic Ca 2+ as interval Fluo-3/FuraRed fluorescence following TCR cross-linking ( B ) or ionomycin induced store-operated calcium flux ( C ) as indicated, captured by flow cytometry. Iono, Ionomycin. n = 3 technical replicates depicted, representative of two independent experiments. D, Representative <t>Phosflow</t> cytometry plots and summary quantification of live, singlet, CD8 + T-cell populations in the presence or absence of soluble TCR cross-linking stimulation for five minutes. E, Concatenated single-cell overlay and colorimetric density tSNE-based depiction of the indicated proteins captured by flow cytometry. F, Representative flow cytometry plots and summary quantification for the indicated markers in ex vivo expanded CD8 + T cells. G, Representative and summary quantification of cytokines following acute TCR restimulation of ex vivo expanded CD8 + T cells. Error bars represent standard deviation. *, P < 0.05; **, P < 0.005; ****, P < 0.001, two-tailed Student t tests ( A, D, F, and G ). ****, P < 0.001 for two-way ANOVA ( B and C ).
Anti Ps6 Af488 P235/P236 Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ps6-af488+antibody/p62+antibody/pm34922003-54-40-44
Average 90 stars, based on 1 article reviews
anti-ps6-af488 p235/p236 antibody - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
cops6 antibody (oti4e7) [alexa fluor® 488]  (Novus Biologicals)
N/A
The COPS6 Antibody (OTI4E7) [Alexa Fluor® 488] from Novus is a COPS6 antibody to COPS6. This antibody reacts with Human, Mouse, Rat. The COPS6 antibody has been validated for the following applications: Western Blot, Immunohistochemistry,
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rps6kc1 antibody (oti10f11) [alexa fluor® 488]  (Novus Biologicals)
N/A
The RPS6KC1 Antibody (OTI10F11) [Alexa Fluor® 488] from Novus is a RPS6KC1 antibody to RPS6KC1. This antibody reacts with Human. The RPS6KC1 antibody has been validated for the following applications: Immunohistochemistry, Immunohistochemistry-Paraffin.
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msk1/rps6ka5 antibody (pcrp-rps6ka5-1a8) [alexa fluor® 488]  (Novus Biologicals)
N/A
The MSK1/RPS6KA5 Antibody (PCRP-RPS6KA5-1A8) [Alexa Fluor® 488] from Novus is a MSK1/RPS6KA5 antibody to MSK1/RPS6KA5. This antibody reacts with Human. The MSK1/RPS6KA5 antibody has been validated for the following applications: Western Blot, Flow Cytometry, Immunocytochemistry/
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Image Search Results


The Na + /K + ATPase restrains tonic signaling and CD8 + T-cell dysfunction. A, Summary bar graphs quantifying fluorescence of the voltage-sensitive indicator DiSBAC 2 3, reflecting the transmembrane electrochemical gradient ( V m ) of CD8 + T cells in standard RPMI-1640 (Vehicle) or isotonic hyperkalemic conditions (K + 20 mmol/L) captured by flow cytometry following CRISPR-Cas9–mediated disruption (Scramble, sgRNA Atp1a1 ) or transduction with retroviral particles. OE, overexpression; DN, Dominant negative. B and C, Summary quantification of cytoplasmic Ca 2+ as interval Fluo-3/FuraRed fluorescence following TCR cross-linking ( B ) or ionomycin induced store-operated calcium flux ( C ) as indicated, captured by flow cytometry. Iono, Ionomycin. n = 3 technical replicates depicted, representative of two independent experiments. D, Representative Phosflow cytometry plots and summary quantification of live, singlet, CD8 + T-cell populations in the presence or absence of soluble TCR cross-linking stimulation for five minutes. E, Concatenated single-cell overlay and colorimetric density tSNE-based depiction of the indicated proteins captured by flow cytometry. F, Representative flow cytometry plots and summary quantification for the indicated markers in ex vivo expanded CD8 + T cells. G, Representative and summary quantification of cytokines following acute TCR restimulation of ex vivo expanded CD8 + T cells. Error bars represent standard deviation. *, P < 0.05; **, P < 0.005; ****, P < 0.001, two-tailed Student t tests ( A, D, F, and G ). ****, P < 0.001 for two-way ANOVA ( B and C ).

Journal: Cancer Immunology Research

Article Title: Intracellular K + Limits T-cell Exhaustion and Preserves Antitumor Function

doi: 10.1158/2326-6066.CIR-23-0319

Figure Lengend Snippet: The Na + /K + ATPase restrains tonic signaling and CD8 + T-cell dysfunction. A, Summary bar graphs quantifying fluorescence of the voltage-sensitive indicator DiSBAC 2 3, reflecting the transmembrane electrochemical gradient ( V m ) of CD8 + T cells in standard RPMI-1640 (Vehicle) or isotonic hyperkalemic conditions (K + 20 mmol/L) captured by flow cytometry following CRISPR-Cas9–mediated disruption (Scramble, sgRNA Atp1a1 ) or transduction with retroviral particles. OE, overexpression; DN, Dominant negative. B and C, Summary quantification of cytoplasmic Ca 2+ as interval Fluo-3/FuraRed fluorescence following TCR cross-linking ( B ) or ionomycin induced store-operated calcium flux ( C ) as indicated, captured by flow cytometry. Iono, Ionomycin. n = 3 technical replicates depicted, representative of two independent experiments. D, Representative Phosflow cytometry plots and summary quantification of live, singlet, CD8 + T-cell populations in the presence or absence of soluble TCR cross-linking stimulation for five minutes. E, Concatenated single-cell overlay and colorimetric density tSNE-based depiction of the indicated proteins captured by flow cytometry. F, Representative flow cytometry plots and summary quantification for the indicated markers in ex vivo expanded CD8 + T cells. G, Representative and summary quantification of cytokines following acute TCR restimulation of ex vivo expanded CD8 + T cells. Error bars represent standard deviation. *, P < 0.05; **, P < 0.005; ****, P < 0.001, two-tailed Student t tests ( A, D, F, and G ). ****, P < 0.001 for two-way ANOVA ( B and C ).

Article Snippet: All antibodies were diluted in 1:200 for staining, except for phosflow antibodies (pS6 S235/6 PE-Cy7, pAKT S473 AF488, pERK T202/Y204 Pacific Blue (Cell Signaling Technology), and pCD3 Y142 PE (BD Biosciences), which were diluted in 1:400 pCD3ζ Y142 PE (BD Biosciences).

Techniques: Fluorescence, Flow Cytometry, CRISPR, Disruption, Transduction, Retroviral, Over Expression, Dominant Negative Mutation, Cytometry, Ex Vivo, Standard Deviation, Two Tailed Test